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Posted: Mon 21 February 2011 - 1 comment(s) [ Comment ] - 0 trackback(s) [ Trackback ]
Category: Health

As my family and I promote our small business, we have recognized an extremely important finding. We have found that “Ganoderma Lucidum” is one of the most exciting medicinal botanical herbs in history. So we decided to put together this bundle of information of studies done by scientist around the world. This information is extracted from an independent source http://www.ncbi.nlm.nih.gov/pubmed?term=ganoderma%20lucidum we hope that you the reader will give our coffee an honest tryout and see if your health improves like the many that have bought our coffee and have return excited with their own testimony about their health improvements. Our coffee, “Ganocafé” is enhanced with Ganoderma Lucidum extract. Ganocafé is the coffee that promotes good health. Salud!

 

Novel medicinal mushroom blend suppresses growth and invasiveness of human breast cancer cells. Jiang J, Sliva D.: Cancer Research Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA.

 

Abstract

Mushrooms are an integral part of Traditional Chinese Medicine (TCM), and have been used for millennia to prevent or treat a variety of diseases. Currently mushrooms or their extracts are used globally in the form of dietary supplements. In the present study we have evaluated the anticancer effects of the dietary supplement, MycoPhyto® Complex (MC), a novel medicinal mushroom blend which consists of a blend of mushroom mycelia from the species Agaricus blazei, Cordyceps sinensis, Coriolus versicolor, Ganoderma lucidum, Grifola frondosa and Polyporus umbellatus, and β-1,3-glucan isolated from the yeast, Saccharomyces cerevisiae. Here, we show that MC demonstrates cytostatic effects through the inhibition of cell proliferation and cell cycle arrest at the G2/M phase of highly invasive human breast cancer cells MDA-MB-231. DNA-microarray analysis revealed that MC inhibits expression of cell cycle regulatory genes (ANAPC2, ANAPC2, BIRC5, Cyclin B1, Cyclin H, CDC20, CDK2, CKS1B, Cullin 1, E2F1, KPNA2, PKMYT1 and TFDP1). Moreover, MC also suppresses the metastatic behavior of MDA-MB-231 by the inhibition of cell adhesion, cell migration and cell invasion. The potency of MC to inhibit invasiveness of breast cancer cells is linked to the suppression of secretion of the urokinase plasminogen activator (uPA) from MDA-MB-231 cells. In conclusion, the MC dietary supplement could have potential therapeutic value in the treatment of invasive human breast cancer. Source: http://www.ncbi.nlm.nih.gov/pubmed/21042722 Ganocafé is infused with the top six, most potent Ganoderma Lucidum species, no other company come close to the health benefits of this coffee.

 

Anti-hepatitis B activities of ganoderic acid from Ganoderma lucidum. Li YQ, Wang SF.: College of Life Science, South China Normal University, Guangzhou 510631, PR China. liyq9168@hotmail.com 

 

Abstract

Ganoderic acid, from Ganoderma lucidum, at 8 microg/ml inhibited replication of hepatitis B virus (HBV) in HepG2215 cells over 8 days. Production of HBV surface antigen and HBV e antigen were 20 and 44% of controls without ganoderic acid. Male KM mice were significantly protected from liver injury, induced with carbon tetrachloride, by treatment with ganoderic acid at 10 mg and 30 mg/kg x d (by intravenous injection) 7 days. Ganoderic acid at the same dosage also significantly protected the mice from liver injury induced by M. bovis BCG plus lipopolysaccharide (from Escherichia coli 0127:B8). Source: http://www.ncbi.nlm.nih.gov/pubmed/16786250 

 

Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. Liu RM, Zhong JJ.: State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.

 

Abstract

In this work, the effects of a pair of positional isomer of ganoderic acids (GAs), namely ganoderic acid Mf (GA-Mf) and ganoderic acid S (GA-S) purified from the fermented mycelia of Ganoderma lucidum, on induction of cell apoptosis and the apoptotic pathway in HeLa cells were investigated. The results demonstrate that both isomers decreased cell population growth on various human carcinoma cell lines by MTT assay, while GA-Mf had better selectivity between normal and cancer cells. The flow cytometry analysis indicated that treatment of HeLa cells with GA-S caused cell cycle arrest in the S phase, while GA-Mf caused cell cycle arrest in the G1 phase. Compared with GA-S, GA-Mf had more potent increase in the number of early and late apoptotic cells. Treatment of HeLa cells with each isomer decreased the mitochondria membrane potential and caused the release of cytochrome c from mitochondria into the cytosol. In addition, stimulation of caspase-3 and caspase-9 activity was observed. The Bax/Bcl-2 ratio was also increased in GA-treated HeLa cells. The results demonstrated that both isomers GA-Mf and GA-S induced apoptosis of human HeLa cells through a mitochondria mediated pathway, but they had the different cell cycle arrest specificity. The findings will be helpful to the development of useful cancer chemopreventive compounds from G. lucidum. Source: http://www.ncbi.nlm.nih.gov/pubmed/21036023

 

GLIS, a bioactive proteoglycan fraction from Ganoderma lucidum, displays anti-tumour activity by increasing both humoral and cellular immune response. Zhang J, Tang Q, Zhou C, Jia W, Da Silva L, Nguyen LD, Reutter W, Fan H.: Institut für Biochemie und Molekularbiologie, CBF, Charité-Universitätsmedizin Berlin, Germany; The Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, PR China.

 

Abstract

AIMS: Ganoderma lucidum, a traditional Chinese medicine, is well known as a modulator of functions of the immune system as well as an anti-tumour agent. However, its active compounds and their molecular mechanisms of action are not well established. GLIS, a proteoglycan isolated from the fruiting body of G. lucidum, stimulates directly the activation of B lymphocytes. In this work, the immunoactivation capacities of GLIS as well as its anti-tumour effect were investigated in vitro and in vivo. MAIN METHODS: Tumour-bearing mice were prepared by inoculation of mouse sarcoma S180 cells into BALB/c mice. Lymphocytes and bone marrow-derived macrophages were isolated from spleen and tibia/femurs, respectively. After stimulation with GLIS different immune responses of these cells were analysed. Anti-tumour effect of GLIS was determined. KEY FINDINGS: After treatment with GLIS, spleen-derived B lymphocytes from tumour-bearing mice became activated, proliferated and produced large amounts of immunoglobulins. Bone marrow-derived macrophages from tumour-bearing mice also became activated after exposure to GLIS, and they produced important immunomodulatory substances, such as IL-1β, TNF-α and reactive nitrogen intermediates, like NO. GLIS markedly increased phagocytosis of macrophages, and very importantly, it markedly raised the macrophage-mediated tumour cytotoxicity. Treatment of mice with GLIS caused an inhibition of mouse sarcoma S180 tumour growth by 60% in vivo.

SIGNIFICANCE: These results indicate that GLIS exhibits a capacity to increase remarkably both humoral and cellular immune activities of tumour-bearing mice and inhibits tumour growth significantly. The anti-tumour effect of GLIS results from its capacity to increase the host's immune activity. Source: http://www.ncbi.nlm.nih.gov/pubmed/20888840

 

Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK). Thyagarajan A, Jedinak A, Nguyen H, Terry C, Baldridge LA, Jiang J, Sliva D.: Methodist Research Institute, Indianapolis, Indiana, USA.

 

Abstract

Medicinal mushroom Ganoderma lucidum is one of the most esteemed natural products that have been used in the traditional Chinese medicine. In this article, we demonstrate that G. lucidum triterpene extract (GLT) suppresses proliferation of human colon cancer cells HT-29 and inhibits tumor growth in a xenograft model of colon cancer. These effects of GLT are associated with the cell cycle arrest at G0/G1 and the induction of the programmed cell death Type II-autophagy in colon cancer cells. Here, we show that GLT induces formation of autophagic vacuoles and upregulates expression of Beclin-1 (1.3-fold increase) and LC-3 (7.3-fold increase) proteins in colon cancer cells and in tumors in a xenograft model (Beclin-1, 3.9-fold increase; LC-3, 1.9-fold increase). Autophagy is mediated through the inhibition of p38 mitogen-activated protein kinase (p38 MAPK) because p38 MAPK inhibitor, SB202190, induces autophagy and expression of Beclin-1 (1.2-fold increase) and LC-3 (7.4-fold increase), and GLT suppresses phosphorylation of p38 MAPK ( approximately 60% inhibition) in colon cancer cells. Taken together, our data demonstrate a novel mechanism responsible for the inhibition of colon cancer cells by G. lucidum and suggest GLT as natural product for the treatment of colon cancer. Source: http://www.ncbi.nlm.nih.gov/pubmed/20574924

 

Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity. Huang SQ, Ning ZX. South China University of Technology, Guangdong Province, Guangzhou, China.

 

Abstract

In the present study, in order to maximize the yield of polysaccharides from Ganoderma lucidum, response surface methodology was employed to optimize the ultrasonic/microwave-assisted extraction (UMAE) conditions. The results indicated that the optimal extraction conditions were ultrasonic power of 50W, microwave power of 284W, extraction time of 701s and water/solid ratio of 11.6:1, respectively. Using UMAE, the yield of polysaccharides was 115.56% above that of classical hot water extraction (HWE) and increased by 27.7% as against ultrasound-assisted extraction (UAE), which confirmed the great potential application of UMAE technology in the extraction of polysaccharides. The immunological assays results demonstrated that polysaccharides of G. lucidum extracted by ultrasonic/microwave (UMP) could improve the weight of immune organ of immunocompromised mice, restore delayed-type hypersensitivity (DTH) reaction to DFNB, improve hemolysis antibody level and natural killer cell activity at high-dose. However, UMP had no noticeable effects on phagocytosis of monocyte at the tested dosage range. Source: http://www.ncbi.nlm.nih.gov/pubmed/20371373

 

Note: There is so much more, but too much to list here. The Coffee I’m promoting has extracts, from the Ganoderma Lucidum mushroom added, with real health benefits. I recommend you try it for yourself. I’m also looking for people that want to build financial freedom with our affiliated business program. It’s easy to sell it, ask me.

 

-Johnny

http://www.myganocafe.com/delectablecafe

http://www.myganocafe.com/sixdegrees

http://www.ncbi.nlm.nih.gov/pubmed?term=ganoderma%20lucidum

This product is not intended to diagnose, treat, cure, or prevent any disease.

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